RT PCR Machine BZPCR-503 offers a robust and user-friendly solution for real-time DNA and RNA amplification and detection. It uses a thermoelectric temperature control system that ensures consistent and accurate thermal performance across all wells. This instrument features multiple fluorescence channels, a long-life LED light source, and a high-sensitivity PMT detector for reliable signal detection. Its intuitive software supports quantification, melting curve, and SNP analysis with automated data processing. Our RT PCR Machine BZPCR-503 with customizable report templates, electric auto hot-lid, and fast scanning capability, offers a seamless and high-throughput workflow for modern laboratories.
| Sample Quantity | 96 well × 0.2ml (optional 96-well×0.1ml) |
| Reaction Volume | 5 to 100 μL |
| Consumable Type | 0.2 mL 96-well plate (half-skirted, non-skirted), 8-strip tubes, Single tube (the bottom should be transparent) |
| Dynamic Range | 1 to 1010 copies |
| Sample Repeatability | CV < 1% |
| Sample Linear | |r| ≥ 0.999 |
| Temperature Control Method | Thermoelectric |
| Temperature Accuracy | ± 0.1℃ |
| Temperature Uniformity | ≤ ± 0.2℃ (55℃) |
| Temperature Gradient Range | 0.1 to 36℃ (Independent temp. control) |
| Hot Lid | Controlled by touch button to automatically press the tube |
| Temperature Range of Hot Lid | RT.+5℃ to 110℃ |
| Ramp Rate (maximum) | 6℃/s |
| Time Increase/Decrease | 0.1 to 9min59s, long PCR is available |
| Temperature Increase/Decrease | 0.1 to 9.9℃, touchdown PCR is available |
| Fluorescence Channel | 5 |
| Fluorescence Dyes | F1: FAM, SYBR Green I, F2: VIC, HEX, TET, JOE, TAMRA, CY3, NED, F3: ROX/TexRed, F4: CY5, F5: CY5.5, F6: Optional |
| Excitation Light Source | LED excitation |
| Excitation Wavelength | Channel 1: 470nm, Channel 2: 523nm, Channel 3: 571nm, Channel 4: 628nm, Channel 5: 678nm |
| Detector | PMT |
| Detection Wavelength | Channel 1: 525nm, Channel 2: 564nm, Channel 3: 612nm, Channel 4: 692nm, Channel 5: 718nm |
| Single Channel Scanning Time | 1.5s |
| Software Function | Support absolute quantitative, relative quantitative, melting curve, HRM, SNP and other functions. |
| Safety Protection and Alarm | Over heat protection and alarm for block and hot lid |
| Data Export | Excel, TXT |
| Interface Mode | USB 2.0/HDMI |
| Voltage Range | 100 to 240V, 50/60 Hz |
| Power | 1000 W |
| Net Weight | 26 Kg |
| Dimension | 473 × 335 × 330 mm |
The RT PCR Machine BZPCR-503 uses fluorescent real-time detection to accurately analyze PCR template amplification. It is ideal for gene research, forensic science, oncology, and ecological biology including zoology and botany. The system supports clinical diagnostics for viruses, tumors, and genetic disorders. Its versatility makes it suitable for both advanced research and routine molecular testing.
Frequently Asked Questions
1: What sample formats are compatible with the BZPCR-503?
Our BZPCR-503 supports standard 0.2 mL 96-well plates (half-skirted and non-skirted), 8-strip tubes, and single tubes with transparent bottoms, providing flexibility for various laboratory workflows.
2: Does the BZPCR-503 support integration with data management systems?
Yes. The BZPCR-503 supports data export in Excel and TXT formats and can be integrated with LIMS systems using customizable report templates.
3: How does the electric hot lid improve user convenience in BZPCR-503?
The electric auto hot-lid is operated by a touch button and automatically presses the tubes, reducing manual steps and ensuring consistent contact during cycling.
4: How does BZPCR-503 perform in multiplexing vs single plex reactions?
The optical system in BZPCR-503 is optimized for high-fidelity multiplex detection without crosstalk between channels, thanks to discrete wavelength excitation and PMT-based detection.
5: How quiet is the BZPCR-503 during operation?
The BZPCR-503 is low-noise, designed for lab environments, with quiet cooling fans and no mechanical scanning mechanisms, unlike older CCD-based systems.
6: What kind of results does the machine provide?
The RT PCR machine provides real-time amplification curves, quantitative data, Ct values, and graphical reports directly on the display or software interface.
7: Can I view real-time results and amplification curves directly on the machine?
Absolutely, the machine displays data results and curve graphs in real-time, allowing immediate analysis without external tools.
8: Does the machine support multiplexing (detecting multiple targets in a single run)?
Yes, multi-channel detection enables multiplex assays, saving time and resources in testing workflows.